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wa09  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc wa09
    Wa09, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 3963 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wa09/WA09/custom%40h9-fi%4010%2E64898%2F2026%2E09%2E09%2E750433
    Average 99 stars, based on 3963 article reviews
    wa09 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Microscopy:

    Article Title: Brain assembloids reveal efficacy and off target effects in high grade glioma brain cancer
    Article Snippet: bryonic; ATCC# CRL-11268) were cultured in DMEM supplemented with 10% FBS and 1% MEM non-essential amino acids. H9/WA09 human embryonic stem cells (H9 hESCs; female, embryonic; Agreement No. 19-W0538; WiCell, USA) were cultured as previously described 21 in feeder-free conditions on vitronectin-coated plates using TeSR-E8 basal medium plus supplement. Stem cells were karyotyped to confirm genomic s

    Biomarker Discovery:

    Article Title: Brain assembloids reveal efficacy and off target effects in high grade glioma brain cancer
    Article Snippet: bryonic; ATCC# CRL-11268) were cultured in DMEM supplemented with 10% FBS and 1% MEM non-essential amino acids. H9/WA09 human embryonic stem cells (H9 hESCs; female, embryonic; Agreement No. 19-W0538; WiCell, USA) were cultured as previously described 21 in feeder-free conditions on vitronectin-coated plates using TeSR-E8 basal medium plus supplement. Stem cells were karyotyped to confirm genomic s



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    wa09  (WiCell Research Institute Inc)
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    WiCell Research Institute Inc wa09
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    https://www.bioz.com/product/wa09/WA09/custom%40h9-fi%4010%2E64898%2F2026%2E09%2E09%2E750433
    Average 99 stars, based on 1 article reviews
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    h9 human escs  (WiCell Research Institute Inc)
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    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human <t>ESCs</t> <t>(H9-EOS).</t> AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .
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    hesc line h9 wa09  (WiCell Research Institute Inc)
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    WiCell Research Institute Inc hesc line h9 wa09
    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human <t>ESCs</t> <t>(H9-EOS).</t> AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .
    Hesc Line H9 Wa09, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wa09/WA09/pmc13108843-218-1-9
    Average 99 stars, based on 1 article reviews
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    Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human ESCs (H9-EOS). AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .

    Journal: iScience

    Article Title: AMPK-p38 axis converts human pluripotent stem cells to naive state

    doi: 10.1016/j.isci.2026.115569

    Figure Lengend Snippet: Naive conversion with AICAR (A) Naive conversion protocol using AICAR. PXGL; PD0325901 (1 μM), XAV939 (2 μM), Go6983 (2 μM), and human LIF (10 ng/mL) in Ndiff227 medium. (B) Flow cytometry analysis of EOS-GFP, SUSD2, CD75, and CD57 expression (day 14). (C) Appearance of EOS-GFP-positive cell clusters induced by AICAR (day 14). (D) EOS-GFP-positive naive-like colony after expansion in PXGL (day 14 + 14p). (E) Flow cytometry analysis of EOS-GFP, SUSD2, and CD75 expression (day 14 + 21p). (F) RT-qPCR analysis of sorted SUSD2 + CD75 + cells and parental primed human ESCs (H9-EOS). AICAR, day 14 + 13p; VPA, day 9 + 4p. Error bars: SD of technical triplicates. (G) Immunostaining for OCT4, NANOG, KLF17, and TFE3 of primed and AICAR-induced cells. AICAR, day 14 + 14p. (H) AICAR-treated cells showed increased TMRE fluorescence, as detected by both flow cytometry (PE channel) and fluorescence microscopy. Scale bars: 100 μm in (C, D, and H) and 50 μm (G). AICAR, day 14 + 16p. See also and and .

    Article Snippet: Primed H9 human ESCs were obtained from WiCell (WA09).

    Techniques: Flow Cytometry, Expressing, Quantitative RT-PCR, Immunostaining, Fluorescence, Microscopy